primary rabbit polyclonal anti-cd14 antibody  (Thermo Fisher)

Journal: Journal of Pain Research

Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

doi: 10.2147/JPR.S135939

Figure Lengend Snippet: Sequences of the primers used in this study

Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

Techniques: Sequencing

Journal: Journal of Pain Research

Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

doi: 10.2147/JPR.S135939

Figure Lengend Snippet: CGRP immunolocalization and mRNA expression in synovial tissue. Notes: Synovial tissue stained with ( A ) DAPI (nuclei), ( B ) CD14 or ( C ) CGRP. ( D ) Merged image. ( E ) High-magnification merged image of ( D ). Scale bars =100 μm. ( F ) Real-time PCR analysis of CGRP expression in non-cultured CD14− and CD14+ fractions. All data are presented as mean ± standard error (n=5). * p <0.05. Abbreviations: CGRP, calcitonin gene-related peptide; DAPI, 4′,6-diamidino-2-phenylindole; PCR, polymerase chain reaction.

Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

Techniques: Expressing, Staining, Real-time Polymerase Chain Reaction, Cell Culture, Polymerase Chain Reaction

Journal: Journal of Pain Research

Article Title: Increase and regulation of synovial calcitonin gene-related peptide expression in patients with painful knee osteoarthritis

doi: 10.2147/JPR.S135939

Figure Lengend Snippet: Effects of PGE2 on CGRP expression in cultured synovial fibroblasts and macrophages. Notes: Synovial fibroblasts and macrophages were stimulated in vitro with PGE2 (10 μM) or without PGE2 (vehicle) for 24 h prior to the extraction of total RNA for ( A ) CD14 and ( B ) CGRP expression analysis. All data are presented as mean ± standard error (n=5). * p <0.05. Abbreviations: PGE2, prostaglandin E2; CGRP, calcitonin gene-related peptide.

Article Snippet: The sections were then treated for 6 h at 4°C with rabbit polyclonal primary antibody against CD14 (Abcam, Cambridge, UK) and mouse monoclonal primary antibody against CGRP (Abcam).

Techniques: Expressing, Cell Culture, In Vitro, Extraction